Diagram of the screening protocol used to assess the biofilm tolerance to antibiotics of Pseudomonas aeruginosa

Diagram of a screening protocolDiagram of the screening protocol used to assess the biofilm tolerance to antibiotics of Pseudomonas aeruginosa. The assay is performed in 96-well plates with a mutant library containing about 6000 mutants; one for each gene in the genome of P. aeruginosa. Here, a side-view of the well is represented to show the behavior of biofilms formed by resistant and sensitive strains. The resistant strain survives the stress imposed by the antibiotic, while the mutant does not. Source: EMPA